RED CELLS Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen
نویسندگان
چکیده
1Molecular Immunology of Parasites Unit, CNRS URA 2581, Institut Pasteur, Paris; 2Radiology Department, Necker Hospital, Assistance Publique des Hopitaux de Paris (APHP), Paris; 3Pharmacology Department, Saint Vincent de Paul Hospital, APHP, Paris; 4Surgery and Pathology Departments, Beaujon Hospital, APHP, Clichy; 5Imaging Platform and 6Histology Unit, Institut Pasteur, Paris; 7Pathology Department, Hôtel Dieu Hospital, APHP, Paris; 8Immunophysiology and Intracellular Parasitism Unit, Institut Pasteur, Paris; and 9Parasitology & Mycology Department, Pitié-Salpêtrière Hospital, APHP, Paris, France
منابع مشابه
Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen.
The current paradigm in Plasmodium falciparum malaria pathogenesis states that young, ring-infected erythrocytes (rings) circulate in peripheral blood and that mature stages are sequestered in the vasculature, avoiding clearance by the spleen. Through ex vivo perfusion of human spleens, we examined the interaction of this unique blood-filtering organ with P falciparum-infected erythrocytes. As ...
متن کاملSurface Area Loss and Increased Sphericity Account for the Splenic Entrapment of Subpopulations of Plasmodium falciparum Ring-Infected Erythrocytes
Ex vivo perfusion of human spleens revealed innate retention of numerous cultured Plasmodium falciparum ring-infected red blood cells (ring-iRBCs). Ring-iRBC retention was confirmed by a microsphiltration device, a microbead-based technology that mimics the mechanical filtering function of the human spleen. However, the cellular alterations underpinning this retention remain unclear. Here, we u...
متن کاملPf155/RESA protein influences the dynamic microcirculatory behavior of ring-stage Plasmodium falciparum infected red blood cells
Proteins exported by Plasmodium falciparum to the red blood cell (RBC) membrane modify the structural properties of the parasitized RBC (Pf-RBC). Although quasi-static single cell assays show reduced ring-stage Pf-RBCs deformability, the parameters influencing their microcirculatory behavior remain unexplored. Here, we study the dynamic properties of ring-stage Pf-RBCs and the role of the paras...
متن کاملThe sensing of poorly deformable red blood cells by the human spleen can be mimicked in vitro.
Retention of poorly deformable red blood cells (RBCs) by the human spleen has been recognized as a critical determinant of pathogenesis in hereditary spherocytosis, malaria, and other RBC disorders. Using an ex vivo perfusion system, we had previously shown that retention of Plasmodium falciparum-infected RBCs (Pf-RBCs) occur in the splenic red pulp, upstream from the sinus wall. To experimenta...
متن کاملApplying a Microfluidic ‘deformability Cytometry’ to Measure Stiffness of Malaria-infected Red Blood Cells at Body and Febrile Temperatures
Red blood cells (RBCs) undergo repeated deformation as they traverse blood vessel, capillaries and splenic cords; RBC deformability is therefore crucial in maintaining normal blood circulation. During falciparum malaria, parasite proteins interact with the spectrin network of host RBCs, moderately stiffening the ring stage infected cells (rings). The subtle modification in the deformability of ...
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تاریخ انتشار 2008